The vibration from different moving structure in car traveling is interactional, judging interactional relations among structures reasonably is premise to analyze the suspension system characteristics. In paper, the transfer characteristics law with the acceleration being exciting are analyzed and summarized, the mount transfer characteristic of the car in driving is derived, and combining with experiment, calculation flow of the mount isolation characteristic is designed, experiments with independent power system excitation test, independent road excitation test, driving test in steady speed are proposed to obtain interference components, common frequency components and the corresponding proportion between vibration source; and finally, by the actual driving testing and data processing, the time-domain isolation characteristics of the power system mounts are obtained. The proposed method can effectively improve the evaluation precision of the mount system and transfer characteristics it is a certain engineering application value.