( College of Marine, Northwestern Polytechnical University, 710072 Xi’an, China )mprove vibration transfer characteristic is an important way to improve ride comfort of motorcycle. Based on the 5-DOF model of motorcycle with road input and Lagrange equation,the frequency response characteristic were obtained for the vertical acceleration on the motorcycle body and displacement of front and rear wheel. With regard to some 125 motorcycles, frequency response characteristic is calculated by using Matlab, and transfer characteristic of motorcycle is tested with motorcycle road simulator. The results show that the analysis and test methods play an important guidance role to improve ride comfort of motorcycle.